What is PCR test for HPV?

Background: The development of a reproducible, sensitive, and standardised human papillomavirus (HPV) polymerase chain reaction (PCR) test is required to implement HPV testing in cervical cancer screening programmes and for triaging women with mild to moderate dysplasia.

What is a Hybrid Capture test?

NAME AND INTENDED USE. ] The HC2 High-Risk HPV DNA Test using Hybrid Capture®2 (HC2) technology is a nucleic acid hybridization assay with signal amplification using microplate chemiluminescence for the qualitative detection of thirteen high-risk types of human papillomavirus (HPV) DNA in cervical specimens.

What is HPV Digene?

Product Details. The digene HC2 HPV DNA Test is an in vitro microplate assay based on signal-amplified nucleic acid hybridization that uses chemiluminescence for the qualitative detection of 18 types of human papillomavirus (HPV) DNA in cervical specimens.

When to Test for HPV?

You may need an HPV test if you: Are a woman aged 30-65. The American Cancer Society recommends women in this age group have an HPV test with a pap smear (co-testing) every five years. If you are a woman of any age that gets an abnormal result on a pap smear.

What is the principle of hybrid capture system?

Principle of Hybrid Capture (HC) HPV Detection Antibodies conjugated with alkaline phosphate and specific for DNA-RNA hybrids react with hybrids which are then subsequently detected using a chemiluminescent substrate. The signal amplification occurs as several conjugated antibodies bind to each hybrid.

What does Hybrid Capture mean?

(hī’brid kap’shŭr) A signal amplification method. An RNA probe is annealed to target DNA; then a captured antibody binds the DNA-RNA hybrid to a solid surface.

How is cobas HPV test done?

The cobas® HPV Test is a qualitative in vitro test for the detection of Human Papillomavirus in cervical specimens collected by a clinician using an endocervical brush/spatula and placed in the ThinPrep Pap Test PreservCyt® Solution or using a cervical broom and placed in SurePath™ Preservative Fluid.

How can I get rid of HPV naturally?

HPV can clear up naturally – as there is no cure for the underlying HPV infection, the only way to get rid of HPV is to wait for the immune system to clear the virus naturally.

When to use QIAGEN hybrid capture 2 kit?

The Hybrid Capture 2 Modular System is intended to be used only in combination with QIAGEN Kits indicated for use with the Hybrid Capture 2 Modular System for applications described in the respective QIAGEN Kit handbooks. Instrument repair service for the Hybrid Capture 2 System at regional repair center.

Which is Digene hybrid capture system version 2?

Amplified chemiluminescent signal detection and result reporting are performed using the DML instrument (see figure ” Luminometer “) with the digene Hybrid Capture System Version 2 (DHCS v.2) Software. To ensure proper calibration, use the LumiCheck Plate with LumiCheck Sotware to monitor the DML instrument performance.

How does the Digene HC2 DNA test work?

The digene HC2 High-Risk HPV DNA Test uses advanced Hybrid Capture 2 technology to directly detect HPV. The digene HC2 HPV DNA Test detects the presence of 18 types of HPV using full genome RNA probes complementary to the HPV DNA, specific antibodies, and chemiluminescent detection.

What is the software for hybrid capture 2?

The software comes on a digene Hybrid Capture 2 System Software CD, which includes the software, digene Hybrid Capture 2 System Assay Protocols, and luminometer plate software. The Microplate Heater I is an electronically controlled heating unit that is designed to accept 96-well microplates when used with digene HC2 DNA Tests.

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