What is epitope tagging used for?
Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. Epitope tags make it possible to detect proteins when no antibody is available. This technique can be used to characterize newly discovered proteins and low abundant proteins.
What is Flag in Western blot?
Flag is a tag (small peptide) that you add to your protein (either to the N or C-terminus) to facilitate the detection or pull down of your protein.
How does a FLAG-tag work?
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence.
How do you use epitope tags?
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The fusion gene is cloned into an appropriate expression vector for the experimental cell type and host cells are transfected.
What is a 3X FLAG tag?
General description. The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).
What is Flag tag antibody?
The FLAG tag (peptide sequence DYKDDDDK) is a short, hydrophilic protein tag commonly used in conjunction with antibodies in protein pull-downs to study protein–protein interactions. We offer quality antibodies specific to FLAG tag that can be used in a variety of research needs.
How big is an HA tag?
The hemagglutinin (HA) tag is a 9-amino acid long peptide corresponding to residues 98-106 of the human influenza HA molecule, an ~63kDa surface glycoprotein required for the infectivity of the human virus.
What is the purpose of chromatin immunoprecipitation?
Chromatin Immunoprecipitation (ChIP) Assay Procedure and Essential Tools. Chromatin immunoprecipitation (ChIP) assays identify links between the genome and the proteome by monitoring transcription regulation through histone modification (epigenetics) or transcription factor–DNA binding interactions.