What is NcoI restriction enzyme?
NcoI (MB066) Description: NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by dam methylation, dcm methylation or CpG methylation. NZYTech’s NcoI is an enzyme that acts at 37 °C.
What are restriction enzymes simple definition?
A restriction enzyme is an enzyme isolated from bacteria that cuts DNA molecules at specific sequences. The isolation of these enzymes was critical to the development of recombinant DNA (rDNA) technology and genetic engineering.
What is a single restriction enzyme?
Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs.
What are restriction enzymes How does HaeIII work?
The enzyme cleaves the DNA at the positions where the GGCC sequence is found. The cleavage occurs between the second and the third nucleotides (G and C). The resulting DNA fragments are known as restriction fragments. HaeIII cuts both strands of DNA in the same location, yielding restriction fragments with blunt ends.
What is the normal function of restriction enzymes?
Restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms.
What is the difference between EcoRI and HindIII restriction enzymes?
The major difference between the two is that EcoRI leaves staggered ends, whereas SmaI leaves blunt ends. Staggered ends are much easier to manipulate during cloning because of the base-pairing capacity inherent in them. (Solution from Diane K. Lavett.)
How big is one unit of BsobI enzyme?
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. BsoBI is a thermophilic isoschizomer of AvaI. Not sensitive to CpG, dcm, or dam methylation.
Which is E coli strain carries the bsob I gene?
An E. coli strain that carries the BsoB I gene from Bacillus stearothermophilus JN2091 (D. Clark). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. BsoBI is a thermophilic isoschizomer of AvaI. Not sensitive to CpG, dcm, or dam methylation.
Why is Neb the leader in restriction enzymes?
Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.